The pGEM ®- T Easy Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments. The pGEM ®- T Easy pre-linearized Vector contains 3´- T overhangs at the insertion site to …
The only difference between pGEM – T and pGEM – T Easy is in the multiple cloning site (MCS). The MCS of the pGEM – T Easy Vector contains sequences on either side of the insert that are recognized by the restriction enzymes Not I and EcoR I. This allows the insert DNA to be removed with a single restriction digest using either of these enzymes.
The pGEM®- T Easy Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments. The pGEM ®- T Easy pre-linearized Vector contains 3´- T overhangs at the insertion site to …
The pGEM ®- T Vector size is 3000bp and the pGEM ®- T Easy Vector size is 3015bp. 6 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516, The pGEM®- T Easy Vector Systems are convienent systems to clone PCR products. They offer all of the advantages of the pGEM ®- T Vector Systems with added convieneice of recognition sites for EcoRI and NotI flankin the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion.
pGEM -T and pGEM -T Easy Vector Systems – Promega, Addgene: Vector Database – pGEM-T Easy Vector, Addgene: Vector Database – pGEM-T Easy Vector, pGEM ®- T Easy Parental vector for TA cloning of PCR products. The insertion site is flanked by BstZI, EcoRI, and NotI sites.
The parent vector is linearized at the position indicated by * in this pGEM®- T Easy Vector Sequence and a T is added at each end. Protocols. Complete Protocol. pGEM®- T and pGEM®- T Easy Vector Systems Technical Manual PDF (548 KB) – English. Quick Protocols.
The pGEM ®- T and pGEM ®- T Easy Vector Systems are convenient systems for the cloning of PCR products. The vectors are prepared by cutting the pGEM ®-5Zf(+) and pGEM ®- T Easy Vectors, respectively, with EcoR V and adding a 3´ terminal thymidine to both ends. These single 3´- T overhangs at the insertion site greatly improve the efficiency …
The pGEM ®- T Vector is derived from the pGEM ®-5Zf(+) Vector (GenBank® Accession No. X65308). The pGEM ®- T Vector was created by linearizing the pGEM ®-5Zf(+) Vector with EcoRV at base 51 and adding a T to both 3´-ends. The position of the T is indicated by * in the pGEM ®- T Vector .
pGEM®- T Easy Vector System????? pGEM ®- T Vector System???????????????????????EcoRI?NotI?????????????????1???NotI?EcoRI????BstZI???????????????????????????DNA???????????????
